Reactivity: | Human, Mouse, Rat |
Applications: | WB, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase |
Gene Symbol: | ABO |
Synonyms: | GTB; NAGAT; A3GALNT; A3GALT1; BGAT |
Gene ID: | 28 |
UniProt ID: | P16442 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 54-354 of human BGAT (NP_065202.2). |
Dilution: | WB 1:500-1:2000 |
Purification Method: | Affinity purification |
Concentration: | 0.50 mg/mL |
Buffer: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-ABO antibody |
Background
This gene encodes proteins related to the first discovered blood group system, ABO. Variation in the ABO gene (chromosome 9q34.2) is the basis of the ABO blood group, thus the presence of an allele determines the blood group in an individual. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene.
Images
![]() | Western blot analysis of lysates from K-562 cells, using BGAT Rabbit pAb (A1586) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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