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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 |
| Gene Symbol: | ACAP2 |
| Synonyms: | CENTB2; CNT-B2; ACAP2 |
| Gene ID: | 23527 |
| UniProt ID: | Q15057 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human ACAP2 (NP_036419.3). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.43 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ACAP2 antibody |
Background
Enables GTPase activator activity. Acts upstream of or within actin filament-based process. Located in ruffle.
Images
 | Western blot analysis of various lysates using ACAP2 Rabbit pAb (A12127) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of U2OS cells using ACAP2 Rabbit pAb (A12127) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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