α-Smooth Muscle Actin (ACTA2) Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A1011
Availability: In Stock

$108.00

Qty

Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, IHC, IF/IC, ELISA
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Polyclonal antibody
Gene Name:	actin alpha 2, smooth muscle
Gene Symbol:	ACTA2
Synonyms:	ACTSA; α-Smooth Muscle Actin (ACTA2)
Gene ID:	59
UniProt ID:	P62736
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human α-Smooth Muscle Actin (ACTA2) (NP_001604.1).
Dilution:	WB 1:500-1:1000; IHC 1:500-1:1000; IF/IC 1:50-1:200
Purification Method:	Affinity purification
Concentration:	0.73 mg/mL
Buffer:	PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-ACTA2 antibody

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.

Images

	Western blot analysis of lysates from Mouse heart, using α-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using α-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using α-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human lung tissue using α-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
	Immunofluorescence analysis of paraffin-embedded rat rectum using α-Smooth Muscle Actin (ACTA2) Rabbit pAb (A1011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.