AGPS Rabbit pAb (20 μl)

AGPS Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A10484
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: alkylglycerone phosphate synthase
Gene Symbol: AGPS
Synonyms: ADAS; ADPS; RCDP3; ADAP-S; ADHAPS; ALDHPSY; AGPS
Gene ID: 8540
UniProt ID: O00116
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 379-658 of human AGPS (NP_003650.1).
Dilution: WB 1:1000-1:2000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 3.66 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-AGPS antibody




Background

This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome.




Images

AGPS antibodyWestern blot analysis of various lysates using AGPS Rabbit pAb (A10484) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using AGPS Rabbit pAb (A10484) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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