Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | aldehyde dehydrogenase 1 family member A1 |
| Gene Symbol: | ALDH1A1 |
| Synonyms: | ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e; ALDH1A1 |
| Gene ID: | 216 |
| UniProt ID: | P00352 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ALDH1A1 (NP_000680.2). |
| Dilution: | WB 1:50000-1:200000; IF/IC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ALDH1A1 antibody |
Background
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
Images
 | Western blot analysis of various lysates, using ALDH1A1 Rabbit mAb (A0157) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of various lysates, using ALDH1A1 Rabbit mAb (A0157) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A549 using ALDH1A1 Rabbit mAb (A0157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of Hep G2 using ALDH1A1 Rabbit mAb (A0157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg ALDH1A1 Rabbit mAb antibody (A0157). Western blot was performed from the immunoprecipitate using ALDH1A1 Rabbit mAb (A0157) at a dilition of 1:20000. |
You may also be interested in:
Related Products
-10%
-16%
