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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | anoctamin 1 |
| Gene Symbol: | ANO1 |
| Synonyms: | DOG1; INDMS; TAOS2; ORAOV2; TMEM16A; ANO1 |
| Gene ID: | 55107 |
| UniProt ID: | Q5XXA6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human ANO1 (NP_060513.5). |
| Dilution: | WB 1:1000-1:2000; IF/IC 1:100-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.09 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ANO1 antibody |
Background
Enables calcium activated cation channel activity; intracellular calcium activated chloride channel activity; and iodide transmembrane transporter activity. Involved in cation transport; inorganic anion transport; and positive regulation of insulin secretion involved in cellular response to glucose stimulus. Located in apical plasma membrane and nucleoplasm.
Images
 | Western blot analysis of various lysates using ANO1 Rabbit pAb (A10498) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using ANO1 Rabbit pAb (A10498) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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