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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | aquaporin 4 |
| Gene Symbol: | AQP4 |
| Synonyms: | MIWC; WCH4; hAQP4; Aquaporin-4 (AQP4) |
| Gene ID: | 361 |
| UniProt ID: | P55087 |
| Clone ID: | 5A4W4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 256-323 of human Aquaporin-4 (AQP4) (NP_001641.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:200-1:800; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 0.62 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-AQP4 antibody |
Background
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon.
Images
 | Western blot analysis of various lysates, using Aquaporin-4 (AQP4) Rabbit mAb (A11210) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb (A11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 (AQP4) Rabbit mAb (A11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aquaporin-4 (AQP4) Rabbit mAb (A11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Aquaporin-4 (AQP4) Rabbit mAb (A11210) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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