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Anti-ATPase B1 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATPase B1 rabbit polyclonal antibody |
Gene Name: | Vacuolar proton pump subunit B |
Synonyms: | V-ATPase subunit B, Atp6v1b1, ATP6B1, VPP3, VATB, VMA2 |
Immunogen: | Recombinant protein corresponding to Mouse ATPase B1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cell membrane |
Uniprot ID: | Q91YH6 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 600-1: 1300 | brain, kidney |
Background
Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain.
Images
| Immunohistochemistry of paraffin embedded mouse kidney using VATPaseB1 (GB111761) at dilution of 1:1000 (300x lens) |
| Immunohistochemistry of paraffin embedded rat brain using VATPaseB1 (GB111761) at dilution of 1:1000 (300x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using VATPaseB1 (GB111761) at dilution of 1:1000 (300x lens) |
| Immunofluorescence of paraffin embedded mouse kidney using VATPaseB1 (GB111761) at dilution of 1:600 (300x lens) |
| Immunofluorescence of paraffin embedded rat kidney using VATPaseB1 (GB111761) at dilution of 1:600 (300x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |