BGAT Rabbit pAb (20 μl)

BGAT Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A1586
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase
Gene Symbol:   ABO
Synonyms:   GTB; NAGAT; A3GALNT; A3GALT1; BGAT
Gene ID:   28
UniProt ID:   P16442
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 54-354 of human BGAT (NP_065202.2).
Dilution:   WB 1:500-1:2000
Purification Method:   Affinity purification
Concentration:   0.50 mg/mL
Buffer:   Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-ABO antibody




Background

This gene encodes proteins related to the first discovered blood group system, ABO. Variation in the ABO gene (chromosome 9q34.2) is the basis of the ABO blood group, thus the presence of an allele determines the blood group in an individual. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene.




Images

ABO antibodyWestern blot analysis of lysates from K-562 cells, using BGAT Rabbit pAb (A1586) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.



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