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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | annexin A2 |
| Gene Symbol: | ANXA2 |
| Synonyms: | P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270; Annexin A2 |
| Gene ID: | 302 |
| UniProt ID: | P07355 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human Annexin A2 (NP_004030.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.15 mg/ml |
| Buffer: | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ANXA2 antibody |
Background
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. Annexin A2 expression has been found to correlate with resistance to treatment against various cancer forms.
Images
 | Western blot analysis of various lysates using Annexin A2 Rabbit pAb (A1572) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of various lysates using Annexin A2 Rabbit pAb (A1572) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Annexin A2 Rabbit pAb (A1572) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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