ARF6 Rabbit pAb (20 μl)

ARF6 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A0988
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: ARF GTPase 6
Gene Symbol: ARF6
Synonyms: ARF6
Gene ID: 382
UniProt ID: P62330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human ARF6 (NP_001654.1).
Dilution: WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 0.55 mg/mL
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-ARF6 antibody




Background

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.




Images

ARF6 antibodyWestern blot analysis of various lysates using ARF6 Rabbit pAb (A0988) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
Immunofluorescence analysis of HeLa cells using ARF6 Rabbit pAb (A0988) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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