ATL2 Rabbit pAb (20 μl)

ATL2 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15884
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   atlastin GTPase 2
Gene Symbol:   ATL2
Synonyms:   aip-2; ARL3IP2; ARL6IP2; atlastin2; ATL2
Gene ID:   64225
UniProt ID:   Q8NHH9
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 390-470 of human ATL2 (NP_001129145.1).
Dilution:   WB 1:500-1:1000; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   1.11 mg/ml
Buffer:   Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-ATL2 antibody




Background

Enables identical protein binding activity. Involved in Golgi organization; endoplasmic reticulum tubular network membrane organization; and protein homooligomerization. Located in endoplasmic reticulum tubular network membrane. Is integral component of membrane.




Images

ATL2 antibodyWestern blot analysis of lysates from HepG2 cells, using ATL2 Rabbit pAb (A15884) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Immunofluorescence analysis of C6 cells using ATL2 Rabbit pAb (A15884) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ATL2 Rabbit pAb (A15884) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using ATL2 Rabbit pAb (A15884) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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