Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | BMI1 proto-oncogene, polycomb ring finger |
| Gene Symbol: | BMI1 |
| Synonyms: | PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1; Bmi1 |
| Gene ID: | 648 |
| UniProt ID: | P35226 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Bmi1 (NP_005171.4). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.85 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-BMI1 antibody |
Background
This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
Images
 | Western blot analysis of various lysates using Bmi1 Rabbit pAb (A0211) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of various lysates using Bmi1 Rabbit pAb (A0211) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunoprecipitation analysis of 200 μg extracts of K-562 cells, using 3 μg Bmi1 antibody (A0211). Western blot was performed from the immunoprecipitate using Bmi1 antibody (A0211) at a dilution of 1:1000. |
You may also be interested in:
Related Products
-10%
-16%
