Reactivity: | Human, Mouse, Rat |
Applications: | WB, IF/IC, ELISA |
Host Species: | Rabbit |
Clonality: | Polyclonal antibody |
Gene Name: | butyrophilin subfamily 3 member A1 |
Gene Symbol: | BTN3A1 |
Synonyms: | BTF5; BT3.1; CD277; BTN3.1; BTN3A1 |
Gene ID: | 11119 |
UniProt ID: | O00481 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human BTN3A1 (NP_008979.3). |
Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:100 |
Purification Method: | Affinity purification |
Concentration: | 3.29 mg/mL |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-BTN3A1 antibody |
Background
The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).
Images
![]() | Western blot analysis of various lysates using BTN3A1 Rabbit pAb (A10288) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
![]() | Immunofluorescence analysis of paraffin-embedded rat lung using BTN3A1 Rabbit pAb (A10288) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of paraffin-embedded mouse lung using BTN3A1 Rabbit pAb (A10288) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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