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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | calcium/calmodulin dependent protein kinase II beta |
| Gene Symbol: | CAMK2B |
| Synonyms: | CAM2; CAMK2; CAMKB; MRD54; CaMKIIbeta; CaMKII |
| Gene ID: | 816 |
| UniProt ID: | Q13554 |
| Clone ID: | 9P2E2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CaMKII (NP_741960.1). |
| Dilution: | WB 1:1000-1:2000; IHC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 0.6 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CAMK2B antibody |
Background
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using CaMKII Rabbit mAb (A0198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Western blot analysis of various lysates using CaMKII Rabbit mAb (A0198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaMKII Rabbit mAb (A0198) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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