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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | CD40 ligand |
| Gene Symbol: | CD40LG |
| Synonyms: | IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L |
| Gene ID: | 959 |
| UniProt ID: | P29965 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 113-261 of human CD40L (NP_000065.1). |
| Dilution: | WB 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 0.38 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD40LG antibody |
Background
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Images
 | Western blot analysis of various lysates using CD40L Rabbit pAb (A0327) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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