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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | cell division cycle 20 |
| Gene Symbol: | CDC20 |
| Synonyms: | CDC20A; OOMD14; p55CDC; OZEMA14; bA276H19.3; CDC20 |
| Gene ID: | 991 |
| UniProt ID: | Q12834 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CDC20 (NP_001246.2?). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.44mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CDC20 antibody |
Background
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.
Images
 | Western blot analysis of lysates from PC-3 cells, using CDC20 Rabbit pAb (A15656) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat ovary using CDC20 Rabbit pAb (A15656) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat spleen using CDC20 Rabbit pAb (A15656) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using CDC20 Rabbit pAb (A15656) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunoprecipitation analysis of 200 μg extracts of LO2 cells using 3 μg CDC20 antibody (A15656). Western blot was performed from the immunoprecipitate using CDC20 antibody (A15656) at a dilution of 1:1000. |
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