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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | cadherin 5 |
| Gene Symbol: | CDH5 |
| Synonyms: | 7B4; CD144 |
| Gene ID: | 1003/12562 |
| UniProt ID: | P33151/P55284 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 259-358 of human VE Cadherin (NP_001786.2). |
| Dilution: | WB 1:2000-1:4000; IHC 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.12mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CDH5 antibody |
Background
This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer.
Images
 | Western blot analysis of various lysates using VE-Cadherin Rabbit PolymAb® (A0734PM)at 1:4000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): HeLa Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using VE-Cadherin Rabbit PolymAb® (A0734PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using VE-Cadherin Rabbit PolymAb® (A0734PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using VE-Cadherin Rabbit PolymAb® (A0734PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using VE-Cadherin Rabbit PolymAb® (A0734PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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