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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 3 subunit A |
| Gene Symbol: | EIF3A |
| Synonyms: | EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta; EIF3A |
| Gene ID: | 8661 |
| UniProt ID: | Q14152 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF3A (NP_003741.1). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 2.72 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-EIF3A antibody |
Background
Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in IRES-dependent viral translational initiation; formation of cytoplasmic translation initiation complex; and viral translational termination-reinitiation. Located in cytosol; nucleolus; and nucleoplasm. Part of eukaryotic translation initiation factor 3 complex.
Images
 | Western blot analysis of lysates from RAW 264.7 cells, using EIF3A Rabbit pAb (A0573) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using EIF3A Rabbit pAb (A0573) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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