Reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | FLII actin remodeling protein |
Gene Symbol: | FLII |
Synonyms: | FLI; FLIL; Fli1; FLII |
Gene ID: | 2314 |
UniProt ID: | Q13045 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1). |
Dilution: | WB 1:200-1:2000; IHC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 1.20 mg/mL |
Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-FLII antibody |
Background
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Images
![]() | Western blot analysis of various lysates using FLII Rabbit pAb (A15273) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5S. |
![]() | Immunohistochemistry analysis of paraffin-embedded Rat heart using FLII Rabbit pAb (A15273) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FLII Rabbit pAb (A15273) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Mouse heart using FLII Rabbit pAb (A15273) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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