GOLGA5 Rabbit pAb (20 μl)

GOLGA5 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15768
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   golgin A5
Gene Symbol:   GOLGA5
Synonyms:   RFG5; GOLIM5; ret-II; GOLGA5
Gene ID:   9950
UniProt ID:   Q8TBA6
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3).
Dilution:   WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   0.75 mg/mL
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-GOLGA5 antibody




Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5.




Images

GOLGA5 antibodyWestern blot analysis of various lysates using GOLGA5 Rabbit pAb (A15768) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunofluorescence analysis of C6 cells using GOLGA5 Rabbit pAb (A15768) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using GOLGA5 Rabbit pAb (A15768) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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