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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | glycogen synthase kinase 3 alpha |
| Gene Symbol: | GSK3A |
| Synonyms: | GSK3 alpha; GSK3A; GSK3α |
| Gene ID: | 2931 |
| UniProt ID: | P49840 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 409-483 of human GSK3α (NP_063937.2). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:20-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 2.01 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GSK3A antibody |
Background
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
Images
 | Western blot analysis of various lysates using GSK3α Rabbit pAb (A0645) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of NIH/3T3 cells using GSK3α Rabbit pAb (A0645) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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