Your shopping cart is empty!
Acetyl-Histone H2B-K12 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA, ChIP |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | H2B clustered histone 5 |
| Gene Symbol: | H2BC21 |
| Synonyms: | H2B; H2BE; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105; H2B-GL105; HIST2H2BE; Acetyl-Histone H2B-K12 |
| Gene ID: | 3017/8349 |
| UniProt ID: | P62807/Q16778 |
| Immunogen: | A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.09 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-H2BC21 antibody |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Images
 | Western blot analysis of various lysates using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at 1:1000 dilution. HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining. |
You may also be interested in:
