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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | hes family bHLH transcription factor 1 |
| Gene Symbol: | HES1 |
| Synonyms: | HHL; HRY; HES-1; bHLHb39; HES1 |
| Gene ID: | 3280 |
| UniProt ID: | Q14469 |
| Clone ID: | 9I2E8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 181-280 of human HES1 (Q14469). |
| Dilution: | WB 1:1000-1:2000; IHC 1:1000-1:4000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.6 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HES1 antibody |
Background
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Images
 | Western blot analysis of various lysates using HES1 Rabbit mAb (A0925) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HES1 Rabbit mAb (A0925) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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