KIFC1 Rabbit mAb (20 μl)

KIFC1 Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A0077
Availability: In Stock
$128.00
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Reactivity: Human, Mouse    
Applications: WB, IHC, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: kinesin family member C1
Gene Symbol: KIFC1
Synonyms: HSET; KNSL2; KIFC1
Gene ID: 3833
UniProt ID: Q9BW19
Clone ID: 10M10J4
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KIFC1 (Q9BW19).
Dilution: WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 0.6 mg/ml
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-KIFC1 antibody




Background

Predicted to enable microtubule binding activity and minus-end-directed microtubule motor activity. Involved in mitotic metaphase plate congression and mitotic spindle assembly. Located in membrane.




Images

KIFC1 antibodyWestern blot analysis of various lysates using KIFC1 Rabbit mAb (A0077) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human appendix using KIFC1 Rabbit mAb (A0077) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using KIFC1 Rabbit mAb (A0077) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit mAb (A0077) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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