Lysozyme (LYZ) Rabbit mAb (20 μl)

Lysozyme (LYZ) Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A0641
Availability: In Stock
$128.00
-+
Reactivity: Human, Mouse    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: lysozyme
Gene Symbol: LYZ
Synonyms: LZM; LYZF1; Lysozyme (LYZ)
Gene ID: 4069
UniProt ID: P61626
Clone ID: 9B2O6
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lysozyme (LYZ) (P61626).
Dilution: WB 1:1000-1:6000; IF/IC 1:200-1:2000
Purification Method: Affinity purification
Concentration: 1.2 mg/mL
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-LYZ antibody




Background

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.




Images

LYZ antibodyWestern blot analysis of various lysates using Lysozyme (LYZ) Rabbit mAb (A0641) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Confocal imaging of paraffin-embedded Human spleen tissue using Lysozyme (LYZ) Rabbit mAb (A0641,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Human lung cancer tissue using Lysozyme (LYZ) Rabbit mAb (A0641,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.



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