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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | mitogen-activated protein kinase kinase kinase 8 |
| Gene Symbol: | MAP3K8 |
| Synonyms: | COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT; MAP3K8 |
| Gene ID: | 1326 |
| UniProt ID: | P41279 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K8 (NP_005195.2). |
| Dilution: | WB 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 0.55 mg/ml |
| Buffer: | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-MAP3K8 antibody |
Background
This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.
Images
 | Western blot analysis of lysates from 293T cells, using MAP3K8 Rabbit pAb (A15623) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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