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| Reactivity: | Human |
| Applications: | WB, IHC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | neutrophil cytosolic factor 4 |
| Gene Symbol: | NCF4 |
| Synonyms: | NCF; CGD3; P40PHOX; SH3PXD4; NCF4/p40-phox |
| Gene ID: | 4689 |
| UniProt ID: | Q15080 |
| Clone ID: | 4L9Z4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 240-339 of human NCF4/p40-phox (Q15080). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NCF4 antibody |
Background
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Images
 | Western blot analysis of lysates from THP-1 cells, using NCF4/p40-phox Rabbit mAb (A0935) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NCF4/p40-phox Rabbit mAb (A0935) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunoprecipitation analysis of 300 μg extracts of THP-1 cells using 3 μg NCF4/p40-phox antibody (A0935). Western blot was performed from the immunoprecipitate using NCF4/p40-phox antibody (A0935) at a dilution of 1:1000. |
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