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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | NFE2 like bZIP transcription factor 2 |
| Gene Symbol: | NFE2L2 |
| Synonyms: | NRF2; HEBP1; Nrf-2; IMDDHH |
| Gene ID: | 4780 |
| UniProt ID: | Q16236 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 243-400 of human NRF2 (NP_006155.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NFE2L2 antibody |
Background
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and NRF2 knockout (KO) HeLa cells using NRF2 Rabbit pAb (A0674) at 1:1000 dilution. wild type (WT) and NRF2 knockout (KO) HeLa cells were treated by MG132(10 μM) at 37℃ for 90 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
 | Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NRF2 Rabbit pAb (A0674) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human placenta using NRF2 Rabbit pAb (A0674) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of Human pancreas tissue using NRF2 Rabbit pAb (A0674) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. |
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