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| Reactivity: | Human |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | nescient helix-loop-helix 1 |
| Gene Symbol: | NHLH1 |
| Synonyms: | HEN1; NSCL; NSCL1; bHLHa35; NHLH1 |
| Gene ID: | 4807 |
| UniProt ID: | Q02575 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human NHLH1 (NP_005589.1). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 3.99 mg/ml |
| Buffer: | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-NHLH1 antibody |
Background
The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).
Images
 | Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NHLH1 using NHLH1 Rabbit pAb (A15695) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of 293F cells transfected with NHLH1 using NHLH1 Rabbit pAb (A15695) at a dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of 293F-NHLH1-Avi-His(C) cells using 3ug NHLH1 Rabbit pAb (A15695 1:300). Western blot was performed from the immunoprecipitate using NHLH1 Rabbit pAb antibody (A15695) at a dilition of 1:3000. |
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