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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | N-ethylmaleimide sensitive factor, vesicle fusing ATPase |
| Gene Symbol: | NSF |
| Synonyms: | SKD2; DEE96; SEC18; NSF |
| Gene ID: | 4905 |
| UniProt ID: | P46459 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 251-400 of human NSF (NP_006169.2). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.48 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NSF antibody |
Background
Enables PDZ domain binding activity and ionotropic glutamate receptor binding activity. Involved in intracellular protein transport; positive regulation of protein catabolic process; and positive regulation of receptor recycling. Located in Golgi apparatus; cytosol; and plasma membrane. Implicated in developmental and epileptic encephalopathy.
Images
 | Western blot analysis of various lysates using NSF Rabbit pAb (A0926) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of BALB-3T3 cells using NSF Rabbit pAb (A0926) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using NSF Rabbit pAb (A0926) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg NSF antibody (A0926). Western blot was performed from the immunoprecipitate using NSF antibody (A0926) at a dilution of 1:3000. |
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