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| Reactivity: | Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | neutral sphingomyelinase activation associated factor |
| Gene Symbol: | NSMAF |
| Synonyms: | FAN; GRAMD5; NSMAF |
| Gene ID: | 8439 |
| UniProt ID: | Q92636 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NSMAF (NP_003571.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.05 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-NSMAF antibody |
Background
This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from mouse brain, using NSMAF Rabbit pAb (A15742) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
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