Your shopping cart is empty!
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | procollagen C-endopeptidase enhancer |
| Gene Symbol: | PCOLCE |
| Synonyms: | PCPE; PCPE1; PCPE-1; PCOLCE |
| Gene ID: | 5118 |
| UniProt ID: | Q15113 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-449 of human PCOLCE (NP_002584.2). |
| Dilution: | WB 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.75 mg/mL |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-PCOLCE antibody |
Background
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.
Images
 | Western blot analysis of lysates from U-251MG cells, using PCOLCE Rabbit pAb (A15298) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
You may also be interested in:
