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PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (100 T)
| Reactivity: | Human |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | PE-Cy7. Ex:565nm. Em:778nm. |
| Gene Name: | interferon gamma |
| Gene Symbol: | IFNG |
| Synonyms: | IFG; IFI; IMD69 |
| Gene ID: | 3458 |
| UniProt ID: | P01579 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFN-gamma (NP_000610.2). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-IFNG Antibody |
Background
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
Images
 | Flow cytometry: 1×10^6 293F cells (negative control,left) and 293F (Transfection,right) cells were intracellularly-stained with PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test,orange line) or PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 293F (Transfection) cells were intracellularly-stained with PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,left) or PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 Human PBMC (untreated,left) andHuman PBMC (treated with 50 ng/mL PMA and 1 ug/mL Ionomycin for 4-6 hours in the presence of monensin,right) were intracellularly-stained with PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test,orange line) or PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 Human PBMC (untreated,left) and Human PBMC (treated with 50 ng/mL PMA and 1 ug/mL Ionomycin for 4-6 hours in the presence of monensin,right) were intracellularly-stained with PE/Cyanine7 Rabbit anti-Human IFN-gamma mAb (A27517,5 μl/Test). |
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