Reactivity: | Human, Mouse, Rat |
Applications: | WB, IF/IC, IP, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | phosphatidylethanolamine binding protein 1 |
Gene Symbol: | PEBP1 |
Synonyms: | PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96; PEBP1 |
Gene ID: | 5037 |
UniProt ID: | P30086 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1). |
Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 0.37 mg/ml |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-PEBP1 antibody |
Background
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome.
Images
![]() | Western blot analysis of various lysates using PEBP1 Rabbit pAb (A0578) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
![]() | Immunofluorescence analysis of HeLa cells using PEBP1 Rabbit pAb (A0578) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of NIH/3T3 cells using PEBP1 Rabbit pAb (A0578) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of PC-12 cells using PEBP1 Rabbit pAb (A0578) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunoprecipitation analysis of 150 μg extracts of MCF-7 cells using 3 μg PEBP1 antibody (A0578). Western blot was performed from the immunoprecipitate using PEBP1 antibody (A0578) at a dilution of 1:500. |
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