Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | phosphoglycerate dehydrogenase |
| Gene Symbol: | PHGDH |
| Synonyms: | NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113; PHGDH |
| Gene ID: | 26227 |
| UniProt ID: | O43175 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 1.11 mg/mL |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PHGDH antibody |
Background
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
Images
 | Western blot analysis of various lysates using PHGDH Rabbit pAb (A10461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using PHGDH Rabbit pAb (A10461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of L929 cells using PHGDH Rabbit pAb (A10461) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of H9C2 cells using PHGDH Rabbit pAb (A10461) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using PHGDH Rabbit pAb (A10461) at dilution of 1:100. Blue: DAPI for nuclear staining. |
You may also be interested in:
Related Products
-10%
-16%
