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PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | protein phosphatase 2 regulatory subunit B'delta |
| Gene Symbol: | PPP2R5D |
| Synonyms: | B56D; MRD35; B56delta; PP2A-B56δ/PR61δ/PPP2R5D |
| Gene ID: | 5528 |
| UniProt ID: | Q14738 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PP2A-B56δ/PR61δ/PP2A-B56δ/PR61δ/PPP2R5D (NP_006236.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.86 mg/mL |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-PPP2R5D antibody |
Background
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
Images
 | Western blot analysis of various lysates using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (A15707) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (A15707) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (A15707) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (A15707) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of L929 cells using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (A15707) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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