Reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC, IF/IC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | protein kinase C delta |
Gene Symbol: | PRKCD |
Synonyms: | MAY1; PKCD; ALPS3; CVID9; nPKC-delta; PKC delta |
Gene ID: | 5580 |
UniProt ID: | Q05655 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PKC delta (NP_006245.2). |
Dilution: | WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:100 |
Purification Method: | Affinity purification |
Concentration: | 1.12 mg/ml |
Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-PRKCD antibody |
Background
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.
Images
![]() | Western blot analysis of various lysates using PKC delta Rabbit pAb (A0471) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min. |
![]() | Immunohistochemistry analysis of paraffin-embedded Rat lung using PKC delta Rabbit pAb (A0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human tonsil using PKC delta Rabbit pAb (A0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Mouse testis using PKC delta Rabbit pAb (A0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
![]() | Immunofluorescence analysis of NIH/3T3 cells using PKC delta Rabbit pAb (A0471) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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