PKC delta Rabbit pAb (20 μl)

PKC delta Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A0471
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IHC, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: protein kinase C delta
Gene Symbol: PRKCD
Synonyms: MAY1; PKCD; ALPS3; CVID9; nPKC-delta; PKC delta
Gene ID: 5580
UniProt ID: Q05655
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PKC delta (NP_006245.2).
Dilution: WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:100
Purification Method: Affinity purification
Concentration: 1.12 mg/ml
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-PRKCD antibody




Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.




Images

PRKCD antibodyWestern blot analysis of various lysates using PKC delta Rabbit pAb (A0471) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
Immunohistochemistry analysis of paraffin-embedded Rat lung using PKC delta Rabbit pAb (A0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using PKC delta Rabbit pAb (A0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using PKC delta Rabbit pAb (A0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using PKC delta Rabbit pAb (A0471) at dilution of 1:100. Blue: DAPI for nuclear staining.



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