RBM39 Rabbit pAb (20 μl)

RBM39 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15760
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IHC, IF/IC, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   RNA binding motif protein 39
Gene Symbol:   RBM39
Synonyms:   HCC1; CAPER; RNPC2; FSAP59; CAPERalpha; RBM39
Gene ID:   9584
UniProt ID:   Q14498
Immunogen:   A synthetic peptide corresponding to a sequence within amino acids 400-508 of human RBM39 (NP_001229528.1).
Dilution:   WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   1.11 mg/ml
Buffer:   Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-RBM39 antibody




Background

This gene encodes a member of the U2AF65 family of proteins. The encoded protein is found in the nucleus, where it co-localizes with core spliceosomal proteins. It has been shown to play a role in both steroid hormone receptor-mediated transcription and alternative splicing, and it is also a transcriptional coregulator of the viral oncoprotein v-Rel. Multiple transcript variants have been observed for this gene. A related pseudogene has been identified on chromosome X.




Images

RBM39 antibodyWestern blot analysis of various lysates using RBM39 Rabbit pAb (A15760) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using RBM39 Rabbit pAb (A15760) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RBM39 Rabbit pAb (A15760) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using RBM39 Rabbit pAb (A15760) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using RBM39 Rabbit pAb (A15760) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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