RNASE6 Rabbit pAb (20 μl)

RNASE6 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15312
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IHC, IF/IC, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   ribonuclease A family member 6
Gene Symbol:   RNASE6
Synonyms:   RAD1; RNS6; RNasek6; RNASE6
Gene ID:   6039
UniProt ID:   Q93091
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human RNASE6 (NP_005606.1).
Dilution:   WB 1:200-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   2.43 mg/mL
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-RNASE6 antibody




Background

The protein encoded by this gene is a member of the ribonuclease A superfamily and functions in the urinary tract. The protein has broad-spectrum antimicrobial activity against pathogenic bacteria.




Images

RNASE6 antibodyWestern blot analysis of lysates from Mouse kidney, using RNASE6 Rabbit pAb (A15312) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using RNASE6 Rabbit pAb (A15312) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human appendix using RNASE6 Rabbit pAb (A15312) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat spleen using RNASE6 Rabbit pAb (A15312) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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