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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | RNA 2',3'-cyclic phosphate and 5'-OH ligase |
| Gene Symbol: | RTCB |
| Synonyms: | FAAP; HSPC117; C22orf28; DJ149A16.6; RTCB |
| Gene ID: | 51493 |
| UniProt ID: | Q9Y3I0 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 236-505 of human RTCB (NP_055121.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.65 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RTCB antibody |
Background
Enables RNA ligase (ATP) activity and vinculin binding activity. Involved in tRNA splicing, via endonucleolytic cleavage and ligation. Located in cytosol and nucleoplasm. Part of tRNA-splicing ligase complex.
Images
 | Western blot analysis of various lysates using RTCB Rabbit pAb (A10305) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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