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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | SIN3 transcription regulator family member A |
| Gene Symbol: | SIN3A |
| Synonyms: | WITKOS; DEL15Q24; CHR15DELq24; Sin3A |
| Gene ID: | 25942 |
| UniProt ID: | Q96ST3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-120 of human Sin3A (NP_056292.1). |
| Dilution: | WB 1:100-1:500; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.51 mg/mL |
| Buffer: | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-SIN3A antibody |
Background
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.
Images
 | Western blot analysis of various lysates using Sin3A Rabbit pAb (A1577) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of U2OS cells using Sin3A Rabbit pAb (A1577). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg Sin3A antibody (A1577). Western blot was performed from the immunoprecipitate using Sin3A antibody (A1577) at a dilution of 1:1000. |
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