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Reactivity: | Human |
Applications: | WB, ELISA |
Host Species: | Rabbit |
Clonality: | Polyclonal antibody |
Gene Name: | transmembrane protein 74B |
Gene Symbol: | TMEM74 |
Synonyms: | NET36; TMEM74 |
Gene ID: | 157753 |
UniProt ID: | Q96NL1 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TMEM74 (NP_694560.1). |
Dilution: | WB 1:500-1:2000 |
Purification Method: | Affinity purification |
Concentration: | 1.01 mg/ml |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-TMEM74 antibody |
Background
Involved in macroautophagy. Predicted to be located in autophagosome membrane; cytoplasmic vesicle; and lysosomal membrane. Predicted to be integral component of membrane.
Images
![]() | Western blot analysis of lysates from DU145 cells, using TMEM74 Rabbit pAb (A10379) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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