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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | UPF1 RNA helicase and ATPase |
| Gene Symbol: | UPF1 |
| Synonyms: | UTF; HUPF1; NORF1; RENT1; smg-2; pNORF1; UPF1/RENT1 |
| Gene ID: | 5976 |
| UniProt ID: | Q92900 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 819-1118 of human UPF1/RENT1 (NP_002902.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 3.56 mg/ml |
| Buffer: | Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-UPF1 antibody |
Background
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using UPF1/RENT1 Rabbit pAb (A1521) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U-2 OS cells using UPF1/RENT1 Rabbit pAb (A1521) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg UPF1/RENT1 antibody (A1521). Western blot was performed from the immunoprecipitate using UPF1/RENT1 antibody (A1521) at a dilution of 1:1000. |
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