WDR18 Rabbit pAb (20 μl)

WDR18 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15875
Availability: In Stock
$108.00
-+
Reactivity:   Human, Rat    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   WD repeat domain 18
Gene Symbol:   WDR18
Synonyms:   Ipi3; R32184_1; WDR18
Gene ID:   57418
UniProt ID:   Q9BV38
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 243-432 of human WDR18 (NP_077005.2).
Dilution:   WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   1.83 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-WDR18 antibody




Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.




Images

WDR18 antibodyWestern blot analysis of various lysates using WDR18 Rabbit pAb (A15875) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.
Immunofluorescence analysis of U-2 OS cells using WDR18 Rabbit pAb (A15875) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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