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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | Wnt family member 3A |
| Gene Symbol: | WNT3A |
| Synonyms: | WNT3A |
| Gene ID: | 89780 |
| UniProt ID: | P56704 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human WNT3A (NP_149122.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.52 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-WNT3A antibody |
Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
Images
 | Western blot analysis of lysates from U2OS cells, using WNT3A Rabbit pAb (A0642) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Western blot analysis of lysates from Mouse Fetal using WNT3A Rabbit pAb (A0642) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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