Adenovirus Ctnnb1 (5×200 μl)

Product Category:

Human Adenovirus expressing mouse Ctnnb1.   

Description:

Pre-made adenovirus for over-expression of mouse Ctnnb1; Ready to ship and ready to use format.

Gene Name:

catenin beta 1

Gene Symbol:

Ctnnb1

Synonyms:

Bfc; Mesc; Catnb

ACCN:

NM_001431671.1

Gene ID:

12387

Vector:

VD053-MAX-CMV-MCS

Fusion Tag:

3×Flag tag

Promoter:

CMV

Titer:

≥1E+10PFU/ml

Storage Buffer:

DMEM with 2.5% BSA, 2.5% glycerol

Background:

The gene Ctnnb1 encodes not only an important cytoplasmic component of the classical cadherin adhesion complex that forms the adherens junction in epithelia and mediates cell-cell adhesion in many other tissues but also a key signaling molecule in the canonical Wnt signaling pathway that controls cell growth and differentiation during both normal development and tumorigenesis. The gene product contains a central armadillo-repeat containing domain through which it binds the cytoplasmic tail of classical cadherins; meanwhile, it also binds alpha-catenin, which further links the cadherin complex to the actin cytoskeleton either directly or indirectly. Beta-catenin is therefore necessary for the adhesive function of classical cadherins. Another key function of this protein is to mediate the canonical Wnt signaling pathway and regulate gene transcription. Without Wnt signal, cytoplasmic beta-catenin that is not associated with the cadherin complex is quickly phosphorylated at the N-terminal Ser/Thr residues by the so called degradation complex containing axin, adenomatous polyposis coli (APC), casein kinase I, and GSK3B, then ubiquitylated by beta-TrCP, and degraded by the proteasome. However, in the presence of Wnt signal, the degradation complex is disrupted and the stabilized cytoplasmic beta-catenin translocates into the nucleus, where it binds various transcription factors and, together with these factors, regulates the transcription of many downstream genes. Mutations of this gene have been linked with various types of tumors. Alternatively spliced variants have been found for this gene.

Storage:

-80°C

Documents:

Manual