Adenovirus Eif4g2 (5×200 μl)

Product Category:

Human Adenovirus expressing mouse Eif4g2.   

Description:

Pre-made adenovirus for over-expression of mouse Eif4g2; Ready to ship and ready to use format.

Gene Name:

eukaryotic translation initiation factor 4, gamma 2

ACCN:

NM_013507

Gene ID:

13690

Vector:

VD053-MAX-CMV-MCS

Fusion Tag:

4×GS & Flag tag

Promoter:

CMV

Titer:

≥1E+10PFU/ml

Storage Buffer:

DMEM with 2.5% BSA, 2.5% glycerol

Background:

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G, that contains the binding sites for eIF4A and eIF3; eIF4G in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. Transgene expression of the apolipoprotein B mRNA-editing enzyme (APOBEC-1) causes extensive editing of this mRNA, which could contribute to the potent oncogenesis induced by overexpression of APOBEC-1. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse. Two alternatively spliced transcript variants that encode different isoforms have been found for this gene.

Storage:

-80°C

Documents:

Manual