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| Product Category: | premade human adenovirus serotype 5 (dE1/E3), FLPo recombinase adenovirus; Ad-CMV-FLPo; Ad-FLPo |
| Description: | FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This Ad-CMV-FLPo pre-packaged adenovirus over-expresses FLPo recombinase under CMV promoter. Ready to use format. |
| Gene: | FLPo recombinase |
| Promoter: | CMV |
| Titer: | 1×1010 ~ 1×1011 PFU/ml |
| Storage Buffer: | DMEM with 2% BSA & 2.5% Glycerol |
| Documents: | Please contact us to request the manual |
