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ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | immunoglobulin heavy constant gamma 2B |
| Gene Symbol: | Ighg2b |
| Synonyms: | IgG2b; Igh-3; gamma2b |
| Gene ID: | 16016 |
| UniProt ID: | P01867-2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 14-113 of mouse IgG2b (P01867-2). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/ml |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Ighg2b antibody |
Background
Enables antigen binding activity. Acts upstream of or within phagocytosis; positive regulation of immune response; and positive regulation of phagocytosis. Located in extracellular space. Part of immunoglobulin complex, circulating. Orthologous to several human genes including IGHG1 (immunoglobulin heavy constant gamma 1 (G1m marker)); IGHG2 (immunoglobulin heavy constant gamma 2 (G2m marker)); and IGHG3 (immunoglobulin heavy constant gamma 3 (G3m marker)).
Images
 | Flow cytometry: 1×10^6 Jurkat cells (negative control,left) and HEL cells (right) were surface-stained with Mouse anti-Human CD235ab mAb (IgG2b, κ,orange line) or Mouse IgG2b isotype control (2 μg/mL,blue line), followed by ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191,5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 HEL cells were surface-stained with Mouse IgG2b isotype control (2 μg/mL,left) or Mouse anti-Human CD235ab mAb (IgG2b, κ,right). ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191,5 μl/Test) was used as a secondary antibody. |
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